Journal: bioRxiv

Article Title: MRN-ATM Pathway Activation in CD4 T-Cell Senescence during Chronic Hepatitis B Virus Infection

doi: 10.64898/2026.03.15.711849

Figure Lengend Snippet: a Telomere length is characterized by MFI of TelC-FITC. b-d Summary data of the FCM for telomeric length of total, naive and memory CD4 T-cell frequencies in PBMCs from patients and HS. e Representative image and summary data of the FCM for the percentages of γ-H2AX level in CD4 T cells. f Representative image and summary data of WB analysis for the level of γ-H2AX in CD4 T cells. g Representative confocal microscopic images and summary data of co-localization of 53BP1 and γ-H2AX in nuclei (DNA damage foci).

Article Snippet: The CD4 T cells were fixed in 2% paraformaldehyde for 20 minutes, permeabilized with 0.3% Triton X-100 in PBS for 10 minutes, blocked with 5% BSA in PBS for 1 hour, and then incubated with rabbit anti-53BP1 antibody (CST) and anti-γ-H2AX (Ser-139) antibody (Biolegend, San Diego, CA, USA) at 4 °C overnight.

Techniques: